Phi Value Analysis Data Formulas

From Foldeomics Wiki

PFD uses formulas for calculating some of the data entered.
Below is a list of formulas used for Phi Value Analysis Data.

Note: In mutant template examples the symbols '$' & '#' refer to wildtype & mutant respectively.

Contents 1 phi F Unfolding 1.1 Kilocalories 1.2 Mutant Template 2 phi F Folding 2.1 Kilocalories 2.2 Mutant Template

phi F Unfolding

Kilocalories

phi F uf = 1-(∆∆G_‡-N(OM) / ∆∆G_D-NH₂O<m>)
phi F uf error = ((∆∆G_‡-N(OM) * ∆∆G_D-NH₂O(m) error)² + (∆∆G_D-NH₂0(m) * ∆∆G_‡-N(OM) error)²)/2)

Mutant Template

BY = 1-(AO/AA)
BZ = ((AO*Z)²+(Y*AP)²)/2

phi F Folding

Kilocalories

phi F f = ∆∆G_‡-D / ∆∆G_D-NH₂O<m>
phi F f error = ((∆∆G_‡-D * ∆∆GD-NH2O(m) error)² + (∆∆G_D-NH₂O(m) + ∆∆G_‡-D error)²)/2

Mutant Template

CA = BB/AA
CB = ((BB*Z)²+(Y*BC)²)/2

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Mark Bate 12:25, 5 September 2006 (EST)