Kinetic Unfolding Data Formulas

From Foldeomics Wiki

PFD uses formulas for calculating some of the data entered.
Below is a list of formulas used for Kinetic Unfolding Data.

Note: In mutant template examples the symbols '$' & '#' refer to wildtype & mutant respectively.

Contents 1 m‡-N 1.1 Kilocalories 1.2 Mutant Template 2 ∆∆G‡-N(OM) 2.1 Kilocalories 2.2 Mutant Template

m_‡-N

Kilocalories

m_‡-N = m_u * (temperature*-0.001986)
m_‡-N error = m_u error * (temperature * -0.001986)

Mutant Template

AM = AK*(K*-0.001986)
AN = AL*(8*-0.001986)

∆∆G_‡-N(OM)

Kilocalories

∆∆G_‡-N(OM) = (temperature*-0.001986) * (lnk_uH₂O wildtype - lnk_uH₂O mutant)
∆∆G_‡-N(OM) error = ✓(lnk_uH₂O error wildtype² + lnk_uH₂O error mutant²)

Mutant Template

AO = (K*-0.001986)*(AI$-AI#)
AP = ✓(AJ$²+AJ#²)

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Mark Bate 10:02, 5 September 2006 (EST)