Kinetic Refolding Data Formulas

From Foldeomics Wiki

PFD uses formulas for calculating some of the data entered.
Below is a list of formulas used for Kinetic Refolding Data.

Note: In mutant template examples the symbols '$' & '#' refer to wildtype & mutant respectively.

Contents 1 m‡-D 1.1 Kilocalories 1.2 Mutant Template 2 ∆∆G‡-D 2.1 Kilocalories 2.2 Mutant Template

m_‡-D

Kilocalories

m_‡-D = m_f * (temperature * -0.001986)
m_‡-D error = m_f error * (temperature * -0.001986)

Mutant Template

AZ = AX*(K*-0.001986)
BA = AY*(K*-0.001986)

∆∆G_‡-D

Kilocalories

∆∆G_‡-D = (temperature * 0.001986) * (lnk_fH₂O wildtype - lnk_fH₂O mutant)
∆∆G_‡-D error = ✓(lnk_fH₂O error wildtype² + lnk_fH₂O error mutant²)

Mutant Template

BB = (K*0.001986)*(AV$-AV#)
BC = ✓(AW$²+AW#²)

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Mark Bate 10:19, 5 September 2006 (EST)